【ACE色譜柱】人參皂苷色譜分析
Ginsenosides from Chinese Medicine by UHPLC-MS/MS

Conditions
Column: ACE UltraCore 2.5 SuperC18
Dimensions: 150 x 3.0 mm
Part Number: CORE-25A-1503U
Mobile Phase: 

A: 0.1% formic acid in H2O
B: 0.1% formic acid in MeCN

Time(mins)	%B
0	25
13	60
15	95
17	95

Flow Rate: 0.4 mL/min
Injection: 2 μL
Temperature: 45 °C
Detection: AB SCIEX 5500 Qtrap MS
ESI in negative ion mode
Source temperature: 450 °C
Sprayer voltage: -4500 V
Stepwise MRM mode for [M + HCOO]- > [M – H]- ion transitions
Mass range 501 – 1250 u (step size 2 u)

1. Ginsenoside Re
(m/z 991 ? 945)
2. Ginsenoside Rg1
(m/z 845 ? 799)
3. Ginsenoside Rf
(m/z 845 ? 799)
4. Ginsenoside Rb1
(m/z 1153 ? 1107)
5. Ginsenoside Rc
(m/z 1123 ? 1077)
6. Ginsenoside Ro
(m/z 1001 ? 955)
7. Ginsenoside Rb2
(m/z 1123 ? 1077)
8. Ginsenoside Rg2
(m/z 829 ? 783)
9. Ginsenoside Rh1
(m/z 683 ? 637)
10. Ginsenoside Rd
(m/z 991 ? 945)
11. Ginsenoside F1
(m/z 683 ? 637)
12. Ginsenoside F2
(m/z 829 ? 783)
13. Ginsenoside Rg3
(m/z 829 ? 783)